VARIATIONS IN DIFFERENT ISOLATES OF Rhizoctonia solani BASED ON TEMPERATURE AND pH
نویسندگان
چکیده
An experiment was conducted to find out variation in isolated Rhizoctonia solani based on radial mycelial growth and sclerotial production. Five isolates of Rhizoctonia solani representing five clusters group were selected and were grown at different levels of temperature and pH on potato dextrose agar (PDA). It was observed that optimum temperature and pH for growth and scierotial production varied among the isolates. The rates of growth and sclerotial formation were not uniform at the same levels of the two growth factors. The maximum mycelial growth of all isolates was found at 30°C. At 35°C, only GAZ-9 and GAZ-18 showed initiation of growth, but the rate was very slow. The optimum temperature for sclerotial production of the isolates GAZ-9, JES16, GAZ-18 SYL-26 was 30°C and for the isolate DIN-8 was 25°C. The optimum pH for maximum radial growth was 6 for DIN-8 and 7 for other four isolates. The maximum number of sclerotia was produced by DIN-8, GAZ-9, and SYL-30 at pH 8, 4, and 7, respectively. The optimum pH for sclerotia formation in JES-16 and GAZ-18 was pH 6.
منابع مشابه
A new acid stable exo--1,3-glucanase of Rhizoctonia solani purified from a commercial source
22 A new acid stable exo--1,3-glucanase of Rhizoctonia solani purified from a commercial source 23 „Kitarase-M‟, by a combination of ammonium sulfate precipitation, ion-exchange and gel filtration 24 methods, had specific activity 0.26 U/mg protein, Km and Vmax values of 0.78 mg/ml and 0.27 mM/min/mg 25 protein, respectively. It had molecular weight of 62 kDa with optimum activity at 40°C temp...
متن کاملIsolation, Identification and Optimal Culture Conditions of Streptomyces albidoflavus C247 Producing Antifungal Agents against Rhizoctonia solani AG2-2
Streptomyces albidoflavus C247 was isolated from the soil of the Gyeongsan golf course in Korea. Physiological, biochemical and 16S rDNA gene sequence analysis strongly suggested that the isolate belonged to Streptomyces albidoflavus. Preliminary screening revealed that the isolate was active against fungi and bacteria. Self-directing optimization was employed to determine the best combination ...
متن کاملIsolation and partial characterization of an antifungal protein produced by Bacillus licheniformis BS-3.
An antifungal protein produced by Bacillus licheniformis strain BS-3 was purified to homogeneity by ammonium sulfate precipitation, DEAE-52 column chromatography and Sephadex G-75 column chromatography. The purified protein was designated as F2 protein, inhibited the growth of Aspergillus niger, Magnaporthe oryzae and Rhizoctonia solani. F2 protein was a monomer with approximately molecular wei...
متن کاملAcaconin, a chitinase-like antifungal protein with cytotoxic and anti-HIV-1 reverse transcriptase activities from Acacia confusa seeds.
From the seeds of Acacia confusa, a chitinase-like antifungal protein designated as acaconin that demonstrated antifungal activity toward Rhizoctonia solani with an IC₅₀ of 30±4 µM was isolated. Acaconin demonstrated an N-terminal sequence with pronounced similarity to chitinases and a molecular mass of 32 kDa. It was isolated by chromatography on Q-Sepharose, SP-Sepharose and Superdex 75 and w...
متن کاملBiological Control of Damping - Off Caused by Pythium ultimum and Rhizoctonia solani with Gliocladium virens in Soilless Mix
Lumsden, R. D., and Locke, J. C. 1989. Biological control of damping-off caused by Pythium ultimum and Rhizoctonia solani with Gliocladium virens in soilless mix. Phytopathology 79:361-366. Gliocladium virens controlled damping-off of zinnia, cotton, and Disease control efficacy lasted for at least 2 mo when G. virens was cabbage caused by Pythium ultimum or Rhizoctonia solani in nonsterile int...
متن کامل